›› 2011, Vol. 38 ›› Issue (9): 65-68.

• 生物技术 • Previous Articles     Next Articles

Cloning and Expression of F1 Gene of Mink CDV Isolate Strain

ZHANG Lei1, CHAI Xiu-li1, ZHANG Da-peng2, ZHANG Hai-ling1, ZHAO Jian-jun1, GAO Han1, BAI Xue1, XU Lei3, YAN Xi-jun1   

  1. 1. Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China;2. Changchun Animal Health Inspection Institute, Changchun 130061, China;3. China Institute of Verterinary Drug Control, Beijing 100081, China
  • Received:2011-01-14 Revised:1900-01-01 Online:2011-09-20 Published:2011-09-20

Abstract: To prepare CDV antigen for diagnosis, gene engeneering method was explored. CDV genome was extracted, F1 gene was amplified by RT-PCR cloned and then sequenced. The F1 gene was cloned into expression plasmid pET28a and expressed by the induction of IPTG inducer. The recombined protein was examined by SDS-PAGE and Western blotting,purified by Ni column. The results indicated the recombined protein could react with CDV positive serum and might be used as CDV diagnostic antigen.

Key words: canine distemper virus; F1 gene; expression

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