Abstract: In an attempt to isolate and identify the epitopes on hemagglutinin(HA) of H1N1 swine influenza virus and to test their antigenicity,polyclonal mouse antisera IgG against recombinant HA was prepared to screen a 12-mer random peptide library. After three rounds of biopanning a phage enrichment was resulted. Forty of the 47 phage clones selected randomly showed to be positive by phage-ELISA and shared 3 amino acid sequences. The result of Western blotting showed that the 3 peptides could bind specially with the polyclonal mouse antisera IgG against H1N1 swine influenza virus indicated that the 3 peptides was antigenicity. In this paper,three antigenic epitopes on HA were obtained and established the foundations for the further research of vaccine and diagnoses of H1N1 swine influenza virus.

Key words: phage displayed peptide library; antigen epitope; swine influenza virus; hemagglutinin