羊布氏杆菌的分离与鉴定

Abstract

Abstract: A gram negative Bacillus pumilus were isolated from a orchiditis goat, it was red in differential staining Coates Pavlovsky, and grew slowly in sheep’s blood agar and chocolate agar. Its 16S rDNA sequence amplification by PCR was 100% identities with Brucella melitensis in GenBank by BLAST, and was strong positive in the agglutination test between the isolated bacterium with the reference Brucella’s antiserum. The two test results shows the isolated bacterium were Brucella melitensis.

Key words: Brucella; 16S rDNA; PCR; identification

CLC Number:

S852.61⁺4

OU Liao-yuan;TAN Zhi-xiang;HE Shi-cheng;HUANG Jian-long;ZHANG Chao-yang;LU Xing-hua;TANG Xiao-ming;WANG Hong-bing. Isolation and Identification of Brucella melitensis[J]. , 2011, 38(8): 231-233.

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Isolation and Identification of Brucella melitensis

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