Abstract: To study the effect of Chinese herbs and Trimethoprim compound on immune function of mouse,and to further disclose the anti-infection mechanism in vivo of Chinese herbs and Trimethoprim compound. Drugs were tested injected into abdominal cavity according to 1,5,10 g/kg body weight for 15 d, the red blood cells,white blood cells,neutrophils,monocytes and lymphocytes were counted in blood of mice by Coulter-JT hematology analyzer. The index of immune organs,peritoneal macrophages phagocytic function,serum lysozyme contents,serum hemolysin levels and lymphocyte proliferation were measured respectively,by using of organ coefficient method,macrophage phagocytic function test,turbidimetry and MTT method. The results showed that Chinese herbs and Trimethoprim compound could significantly increase the number of red blood cells,the proportion of monocytes in mice (P＜0.05). Chinese herbs and Trimethoprim compound could significantly increase the index of immune organs,enhance peritoneal macrophage phagocytosis and serum lysozyme activity,and improve the level of serum hemolysin and lymphocytes proliferative capacity(P＜0.05). The non-specific and specific immune function in mice were enhanced by Chinese herbs and Trimethoprim compound.

Key words: Chinese herbs; Trimethoprim; mouse; immune indicators; hematology indicators