Abstract: In view of the difficulty of teeth of morphological study, chose the proper reagent for fixed, and the appropriate decalcify solution （EDTA-2Na） for completing the paraffin sections of teeth. The teeth histological sections were carried out using Masson trichrome staining and HE staining, by determining the advisable procedure and staining time firstly. Compared with HE staining, the main components were stained with different colours for bright and contrasted more obvious in Masson trichrome staining, which would paly an important role on the physiology and pathological morphological studies of teeth. For the similar characteristics of hard tissues in the body, this method could be referenced, improved and applied in the bone, cartilage and other hard tissues.

Key words: HE staining; Masson trichrome staining; paraffin section; dental tissues; decalcification; collagen fibers