Abstract: Monoclonal antibody play an important role in the fields of modern bioinstrumentation and medical treat. Regardless of the subtypes and the forms of sauce, any antibody should be purified before being used to detect and therapy. The methods of purification are different by the kinds and purpose of antibodies. They can be divided into two types, sedimentation and chromatography. This paper review these methods by the range of application, purity of output, coefficient of recovery, cycle of purification, batch of samples and cost.

Key words: monoclonal antibody; purification; sedimentation; chromatography