鹅细小病毒单克隆抗体的制备与鉴定

Abstract

Abstract: In order to prepare the monoclonal antibody of goose parvovirus, BALB/c mice were immunized by concentrated GPV and anti-GPV hybridoma cells（4B4） were obtained by hybridoma technique, indirect ELISA and limiting dilution assay. After identification, the heavy chain of McAb was IgG1 subtype and the light chain was κ chain. The results of ELISA and Western blotting showed that GPV and GPV-VP3 protein could be specially recognized by 4B4 McAb. It indicated that the epitope recognized by 4B4 McAb might be the conservated region of GPV VP3 protein. This research settled the foundation for the characterization of GPV epitope and the investigation of GPV diagnostic reagent.

Key words: goose parvovirus; monoclonal antibody; VP3 protein

CLC Number:

S852.65

DU Qiu-ming;LU Cheng;GAO Jian-wei;ZHAO Wen-jing;SHEN Feng-yong;MENG Qi-lin. Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody[J]. , 2011, 38(2): 162-164.

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Preparation and Evaluation of Goose Parvovirus Monoclonal Antibody

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