›› 2011, Vol. 38 ›› Issue (12): 76-78.

• 生物技术 • Previous Articles     Next Articles

Isolation and Identification of Mycoplasma bovis Xinjiang Strain Inducing Pneumonia and Arthritis of Calf

YAO Yong-jin1, YAN Gen-qiang1, WANG Jing-mei1, FAN Wei-xing2, LI Yan3   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003,China;2. China Animal Health and Epidemiology Center, Qingdao 266032, China;3. Production and Construction Corps Animal Husbandryand Veterinary Primal Station, Urumqi 830052, China
  • Received:2011-05-05 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract: Since July 2010, a serious pneumonia and arthritis occurred in the calves of some dairies in north area of Xinjiang. In order to detect the pathogen, the died calf lungs and joint fluid, the sick calf serum and some nasal swabs was collected with asepsis from 8 dairies. Add the samples was inoculated the selected material medium. The 15 Mycoplasma bovis(M.bovis) isolates were identified by colony morphology, specific PCR, growth inhibition test (GIT) and serological test, 7 isolates were cloned and sequenced, the homology of nucleotide sequences were 97.32% to 100.00% in OPP F gene with PG45 strain. Furthermore, 92 serum samples were detected by ELISA, and the positive rate was 82.61%. The results indicated that the disease was primarily caused by M.bovis.

Key words: calf; pneumonia; arthritis; Mycoplasma bovis; isolation; identification

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