›› 2011, Vol. 38 ›› Issue (12): 142-144.

• 遗传繁育 • Previous Articles     Next Articles

Polymorphism in PLIN Gene Intron 5 and its Association with Carcass and Fatness Traits in Chicken

LEI Qiu-xia, ZHOU Yan, HAN Hai-xia, LI Gui-ming, CAO Ding-guo, LI Fu-wei, LU Yan   

  1. Institute of Poultry Science, Academy of Agricultural Sciences of Shandong Province, Jinan 250023, China
  • Received:1900-01-01 Revised:2011-08-10 Online:2011-12-20 Published:2011-12-20

Abstract: The genetic polymorphisms in perilipin(PLIN) gene were detected by PCR-RFLP method in five Shandong local chickens and one breeding strain including Jining Bairi chicken, Wenshang Luhua chicken et al, the correlations between the SNP loci and the carcass and fatness traits were analyzed. As a result, an G→T mutation of Pst Ⅰ restricton site at 2224 bp in PLIN gene was firstly identified in the six populations. Two genotypes (A1A1 and A1A2) were detected, and the allele A1 was predominant in all the six experimental populations. The statistical analysis showed that the 2224 bp polymorphism locus was not association with carcass and fatness traits (P>0.05)。The chickens with A1A1 genotype had higher carcass and fatness trait means than chickens with A1A2 genotype, but no significant difference was found between them (P>0.05). The results of this research showed that the 2224 bp polymorphism locus in chicken PLIN wasn't an effective marker for carcass and fatness traits in chickens.

Key words: chicken; PLIN gene; PCR-RFLP; carcass traits; fatness traits

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