Abstract: This research was conducted to determine the effect of different hormones or treatments methods on lamb's follicular oocytes development, and the difference of oocytes' maturation process in vitro between superovulated lamb and normal adult ewe was studied.Besides, the maturing rate of lamb's oocytes in vitro based on different prescription of culture solution was determined. The results suggested as following: the optimal age for early using of Liaoning Cashmere goats' lamb was 5 to 6 weeks, with the body weight between 5.6 to 7.5 kg, the optimal method of lamb's superovulation was injecting FSH 40 mg three times and PMSG 400 IU once in sequence with the interval of 12 hours, adding 10% FBS (fetal bovine serum) into culture solution can be replaced by 12 mg/mL BSA, which influenced nothing to the maturation of lamb's oocytes. However, the matured rate of oocytes in vitro of superovulated lamb's was significantly lower than normal adult ewe's.

Key words: female lamb; follicular development; oocyte; matured in vitro