羊魏氏梭菌PCR方法的建立及初步应用

›› 2011, Vol. 38 ›› Issue (11): 106-108.

• 生物技术 • Previous Articles Next Articles

Establishment and Application of PCR Assay for Clostridium perfringens Isolated from Sheep

YU Bo¹, RAN Mao-tao¹, TAN Shi-wen¹, XU Jing-e¹, WEI Ci-kai², AI Yu-ping¹

1. Guizhou Institute of Animal Husbandry and Veterinary Medicine,Guiyang 550005,China;2. Weng'an County Animal Disease Control Center,Weng'an 550400,China

Received:2011-04-02 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract

Abstract: According to the gene sequences in GenBank of alpha-toxin,one pair of specific primer was designed for amplifying the specific fragments of alpha-toxin gene. After optimization of annealing temperature and primers concentrations,PCR assay was established for simultaneous detection of the Clostridium perfringens. The specific bands of 255 bp was amplified.The sensitivity and specificity tests showed that the PCR were sensitive in 0.39,0.62,0.52,0.87,0.92 ng/L A,B,C,D,EC.perfringens. No band was amplified from Escherichia coli,Streptococcus,Staphyloccocus aureus and Pasteurella multocida. 54 clinical samples were detected by PCR.The results revealed that the established PCR assay was sensitive,specific and reproducible and it could be used to detect rapidly Clostridium perfringens isolated from sheep.

Key words: Clostridium perfringens isolated from sheep; alpha-toxin gene; rapid diagnosis

CLC Number:

S858.26

YU Bo;RAN Mao-tao;TAN Shi-wen;XU Jing-e;WEI Ci-kai;AI Yu-ping. Establishment and Application of PCR Assay for Clostridium perfringens Isolated from Sheep[J]. , 2011, 38(11): 106-108.

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Establishment and Application of PCR Assay for Clostridium perfringens Isolated from Sheep

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