›› 2010, Vol. 37 ›› Issue (8): 34-36.

• 生物技术 • Previous Articles     Next Articles

Screening Proteins Interacting with PrP23-231 Using Yeast Two-hybrid System

SONG Bo-cui1,2,CHEN Zhi-bao1,WAN Jia-yu2,GAO Hong-wei2   

  1. (1.Heilongjiang Bayi Agricultural University,Daqing 163319,China;2. Research Institute of Veterinary,Academy of Military Medical Sciences,Changchun 130062,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
  • Contact: GAO Hong-wei

Abstract: The mouse leukocyte cDNA library was screened with pSos-PrP23-231 as bait plasmid by yeast two hybrid system,then the positive yeast clones were obtained. The candidate gene were confirmed by DNA sequencing and BLAST analysis in NCBI,the gene sequence were obtained with their coding protein interacting with pSos-PrP23-231,this candidate genes possibly involve with pathogenesis of BSE.

Key words: prion; yeast two-hybrid system; protein interaction

CLC Number: