胶体金标记抗圆弧青霉菌毒素—青霉酸单克隆抗体的研究

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (8): 30-33.

• 生物技术 • Previous Articles Next Articles

Study on Colloidal Gold Labeled Monoclonal Antibody against Penicillic Acid from Penicillium cyclopium

ZHU Li,LEI Hong-yu, YUAN Hui

(College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20
Contact: YUAN Hui

Abstract

Abstract: Preparative method of colloidal gold labeled monoclonal antibody against penicillic acid (PA) from Penicillium cyclopium was established and its reaction condition was optimized. The activity of the gold-labeled probe was determinated. Results showed that the optimization pH
was 8.2, the minimum proteins labeled 1 mL colloidal gold was 25 μg. It showed that the probe and anti-mouse anti-goat immnoglobulins were compaginated. The study provided foundation for colloidal gold immunochromotography assay against PA.

Key words: colloidal gold; monoclonal antibody against penicillic acid; gold-labeled probe

CLC Number:

S859.79⁺7

ZHU Li;LEI Hong-yu;YUAN Hui. Study on Colloidal Gold Labeled Monoclonal Antibody against Penicillic Acid from Penicillium cyclopium[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(8): 30-33.

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Study on Colloidal Gold Labeled Monoclonal Antibody against Penicillic Acid from Penicillium cyclopium

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