猪源支气管败血波氏杆菌河南株的分离鉴定

›› 2010, Vol. 37 ›› Issue (7): 146-148.

• 疾病防治 • Previous Articles Next Articles

The Isolation and Identification of Swine Bordetella bronchiseptica

ZHANG Jia-ming1，ZUO Song1，TAO Jia-quan2，ZHANG Xu-ke1,2

(1.College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;2.National Engineering & Technology Research Center for Veterinary Medicine,Luoyang 470003, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-07-20 Published:2010-07-20
Contact: ZHANG Xu-ke

Abstract

Abstract: 126 portions of the lung tissue samples or nasal swabs with respiratory symptoms, collected from number of pig farms in Henan province, were used to isolate and identify Bordetella bronchiseptica(Bb).According to bacterial culture characteristics, Gram staining, biochemical tests and PCR, 11 strains Bb have been successfully isolated. Three important virulent factors(fha,prn,dnt) for Bb were tested by PCR, most of the PCR results obtained corresponding DNA target bands except that two strains Bb didn’t get prn gene target bands. Erythrocyte agglutination assay showed all kinds of strains were able to agglutinate pig and sheep’s erythrocyte at different degree. Skin necrosis in suckling mice showed all kinds of strains were able to produce skin necrosis to different extent. Virulent strains(HN0610, HN0806, HN0922, HN0827) were confirmed by mouse lethal.

Key words: Bordetella bronchiseptica; isolation and identification; virulent factor; PCR

CLC Number:

S858.28

ZHANG Jia-ming;ZUO Song;TAO Jia-quan;ZHANG Xu-ke;. The Isolation and Identification of Swine Bordetella bronchiseptica[J]. , 2010, 37(7): 146-148.

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The Isolation and Identification of Swine Bordetella bronchiseptica

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