梅花鹿促卵泡激素α亚基基因克隆与融合表达

Abstract

Abstract: FSHα-subunit gene segments were amplified by PCR from the original constructed vector. Then the PCR product was TA cloned, sequenced and inserted into the carrier pGEX-6P-2. The recombinant plasmids were identified by restricted enzymes digestion, and then the positive clones were transformed into E.coli BL21. GST-FSHα fusion proteins were expressed via the induction of IPTG, and detected by SDS-PAGE. The results showed that PCR product was about 380 bp,and sequencing result was identical with GenBank. The pGEX-6P-2-FSHα positive clone produced a 39.3 ku fusion protein on SDS-PAGE gel. Successfully cloning and expressing Cervus nippon FSHα-subunit gene.

Key words: FSHα-subunit gene; clone; fusion expression

CLC Number:

ZHANG Jian-xiao;GUAN Hong-bin. Cloning and Fusion Expression of FSHα-subunit Gene in Cervus Nippon[J]. , 2010, 37(6): 59-62.

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Cloning and Fusion Expression of FSHα-subunit Gene in Cervus Nippon

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