犬型钩端螺旋体外膜蛋白OmpL1基因的克隆与序列分析

›› 2010, Vol. 37 ›› Issue (6): 55-58.

• 生物技术 • Previous Articles Next Articles

Cloning and Analysis of the Sequence of Outer Membrance Protein Gene ofa Serovar Leptospire canicola Interrogans

Fang-liang1, FAN Quan-shui2, QIU Wei2, YIN Ge-fen3, XING Yun1

（1.Kunming Police-dog Base of the Ministry of Public Security, Kunming 650204, China; 2.Centre for Disease Prevention and Control, Chengdu Military Region, Kunming 650032, China; 3.Yunnan Agricultural University, Kunming 650201, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
Contact: FAN Quan-shui

Abstract

Abstract: In this study, the OmpL1 was amplified by PCR from a serovar Leptospire canicola interrogans, and it was cloned into plasmid pVAX1,then sequence analysis was done. These all got an expectation about the 960 bp of special tape, it’s sequence analysis and gene comparision with Leptospire hebdomadis,Leptospire autumnalis,Leptospire pomona and Leptospire australis, the homology of nucleotide sequences was 99.38%，93.77%，99.58% and 99.38%, the homology of deduced amino acid was 100%，95%，100% and 100%. It indicated that OmpL1 was a conserved antigen among the pathogenic Leptospira species, fusion protein OmpL1 might play an important role in diagnosis and prevention of Loptosirosis.

Key words: Leptospire canicola; outer membrance protein gene; OmpL1 gene; clone; sequence analysis

CLC Number:

Fang-liang;FAN Quan-shui;QIU Wei;YIN Ge-fen;XING Yun. Cloning and Analysis of the Sequence of Outer Membrance Protein Gene ofa Serovar Leptospire canicola Interrogans[J]. , 2010, 37(6): 55-58.

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Cloning and Analysis of the Sequence of Outer Membrance Protein Gene ofa Serovar Leptospire canicola Interrogans

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