›› 2010, Vol. 37 ›› Issue (6): 47-49.

• 生物技术 • Previous Articles     Next Articles

Sequential Culture of Porcine Parthenogenetic Embryos

ZHANG Li-ping1， WANG Ya-gang2，3， LI Li2，3， LIU Xi-mei2，3， QIAO Xian-feng2，3， ZHENG Xin-min2，3， XU Sheng-cheng1   

  1. （1.Agriculture and Animal Husbandry College，Qinghai University，Xining 810016，China;2.Institute of Animal and Veterinary Science，Hubei Academy of Agricultural Science，Wuhan 430064，China;3.Hubei Lab of Animal Embryo and Molecular Breeding ，Wuhan 430064，China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-06-20 Published:2010-06-20
  • Contact: ZHENG Xin-min；XU Sheng-cheng

Abstract: The purpose of the present study was to examine the effect of different sequential cultures on the porcine parthenogenetic embryos of development after activation. The ordinary culture and sequential culture were used in vitro culture of procine oocytes. The results showed that NCSU-23 supplemented 0.4% BSA was a better culture media for the oocytes development after activated in the early stage, but mTCM-199 supplemented 0.1% PVA facilitates parthenogenetic embryos breakthrough 4-cell block and reach morula；the sequential cultures system of NCSU-23 supplemented 0.4% BSA and mTCM-199 supplemented 0.1 % PVA could improve the development of porcine parthenogenetic embryos.

Key words: porcine; parthenogenetic embryos; sequential culture

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