16S rRNA甲基化酶rmtB在猪肠道菌中的传播方式分析

›› 2010, Vol. 37 ›› Issue (3): 55-59.

• 生理生化 • Previous Articles Next Articles

Transfer Mode of 16S rRNA Methylase rmtB in EnterobacteriaIsolates from Pig Farms

CHEN Lin^1,2, ZHANG Jun-feng³, LIU Jian-hua¹, CHEN Zhang-liu¹, ZENG Zhen-ling¹

(1.Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2.Jiangsu Animal Science and Veterinary Medicine College, Taizhou 225300, China; 3.Chongqing Academy of Animal Science, Rongchang 402460, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20

Abstract

Abstract: To study the transfer mode of rmtB in Enterobacteria isolates from pig farms. Pulse field gel electrophoresis（PFGE） were carried out to study the genotyping of 48 rmtB-positive E.coli isolates from A and B pig farms. Conjugation experiments was carried out to study the horizontal transfer mode of rmtB genes by filter mating method. Broth microdilution method was applied to test susceptibility of rmtB-positive isolates and their transconjugants to antimicrobial agents. Among 48 rmtB-positive E.coli isolates, 45 strains were successfully typed by PFGE. Total of 28 different PFGE profiles were obtained, and 12 different PFGE profiles were obtained in 20 isolats from A pig farm, 16 different PFGE profiles were acquired in 25 isolats from B pig farm. All the rmtB genes located in 46 rmtB-positive isolates were easily transferred to recipients of E.coli C600 and E.coli 488 Rifr. Conjugative transfer frequencies varied from 4.6×10^-13 to 3.0×10^-6 cells per recipient cell. And all 46 transconjugants showed extraordinarily high-level resistance to 4,6-substituted deoxystreptamine (MICs>512 μg/mL). It concluded that the rmtB gene was located on a conjugative plasmid in E.coli. Both horizontal gene transfer and clonal expansion were responsible for the dissemination of rmtB gene and horizontal gene transfer was predominant in E.coli isolates from two pig farms.

Key words: 16S rRNA methylase; rmtB; plasmid; horizontal gene transfer; clonal expansion

CLC Number:

S859.7

CHEN Lin;ZHANG Jun-feng;LIU Jian-hua;CHEN Zhang-liu;ZENG Zhen-ling. Transfer Mode of 16S rRNA Methylase rmtB in EnterobacteriaIsolates from Pig Farms[J]. , 2010, 37(3): 55-59.

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Transfer Mode of 16S rRNA Methylase rmtB in EnterobacteriaIsolates from Pig Farms

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