猪源携带质粒介导喹诺酮类耐药基因大肠杆菌气源性传播的研究

doi:10.16431/j.cnki.1671-7236.2015.07.032

Abstract

Abstract: To investigate the aerotransmission of Escherichia coli (E.coli) carrying the plasmid-mediated quinolone resistance (PMQR) genes,airborne E.coli were isolated from indoor air,upwind air and downwind air samples in five swine farms.Fecal samples from swine houses were randomly collected to isolate the E.coli.The sensitivities of the E.coli strains against 14 antibiotics were tested.The E.coli carrying the PMQR genes (qnr,aac(6')-Ib-cr,qepA) were identified by ERIC-PCR,and then the genetic fingerprints of E.coli were established to analyze its origins and spread toward the outside surroundings.The results showed that E.coli isolated from five swine farms showed high resistance against 12 antibiotics,such as gentamicin,kanamycin,tetracycline,streptomycin,nalidixic acid and sulfamethoxazole,and presented multi-drug resistant.Results of ERIC-PCR showed that 46.34% (19/41) of strains isolated from indoor air samples had the same origin with fecal-obtained strains,and 73.68% (14/19) of them shared the same PMQR genes with fecal-obtained strains.68.42% (26/38) of strains isolated from downwind air samples had the same origin with fecal-obtained or indoor air-obtained strains,and 65.38% (17/26) of them shared the same PMQR genes with fecal-obtained or indoor air-obtained strains.This indicated that E.coli carrying PMQR genes and originating from feces in swine houses could form aerosols to pollute the indoor air and then spread to the downwind air through air exchange (≥400 m),which could be a potential threaten to public environment and human health.

Key words: E.coli aerosols; resistance status; plasmid-mediated quinolone resistance (PMQR) genes; genetic similarity; public health

CLC Number:

S852.61

ZHANG Xiao-dan, GAO Li-li, HU Jia-qing, AI Wen-hao, CHAI Tong-jie. Study on Aerotransmission of Escherichia coli Carrying the Plasmid-mediated Quinolone Resistance Genes of Swine[J]. , 2015, 42(7): 1842-1850.

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Study on Aerotransmission of Escherichia coli Carrying the Plasmid-mediated Quinolone Resistance Genes of Swine

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