斑点杂交法在鸡毒支原体污染检测中的应用

Abstract

Abstract: In this experiment, a DNA probe was designed and DIG-labeled by the predecessors according to the 16S rRNA gene sequence of M.gallisepticum（MG） published in GenBank. The positively charged nylon membranes were used in the Dot-blotting analysis. This method was applied to detect the samples of Newcastle disease live vaccines. The results showed that the MG contamination can be detected in the live vaccines by Dot-blotting hybridization. The rate of detection was 23.3% (7/30). The Dot-blotting hybridization was a sensitive and specific method in the Mycoplasma contamination detection of biologicals. 

Key words:  M.gallisepticum; DNA probe; Dot-blotting hybridization

CLC Number:

S852.62

MA Yan-qin;ZHANG Ying. Application of Dot-blotting Hybridization Technique on the Detection of M.gallisepticum Contamination in Live Vaccine[J]. , 2010, 37(3): 201-203.

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Application of Dot-blotting Hybridization Technique on the Detection of M.gallisepticum Contamination in Live Vaccine

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