牛支原体新疆分离株oppD/F基因克隆及分子特征分析

doi:10.16431/j.cnki.1671-7236.2020.01.003

Abstract

Abstract: This study was aimed to clone and analyze the oppD/F gene of Mycoplasma bovis isolated strain in Xinjiang,explore the sequence characteristics of oppD/F gene and the structure and function of its encoded protein.One pair of specific primers was designed based on the sequence of oppD/F gene of PG45 strain (accession No.:AF130119.1) in GenBank,the sequence of oppD/F gene was amplified by PCR,and it was inserted into pMD19-T vector for sequencing.Its nucleotide sequence,amino acids sequence,the basic physicochemical properties,hydrophobicity,soluble,signal peptide,transmembrane,subcellular localization,phosphorylation site,glycosylation site,secondary structure,tertiary structure,function of oppD/F protein were predicted and analyzed by bioinformatics methods.The results showed that the sequence length of Mycoplasma bovis isolated strain oppD/F gene was 1 617 bp,the sequence and phylogenetic tree analysis indicated that it showed 100% homology with that of Mycoplasma bovis strain PG45 and Mycoplasma bovis isolate JF4278,its were closely relative which were in the same branch.The oppD/F protein encoded a protein composed of 539 amino acids,no signal peptide and transmembrane structure,it was a stable soluble hydrophilic protein.The oppD/F protein had a AAA super family domain,50 potential phosphorylation sites and 3 glycosylation sites.The secondary structure was the mixed,and the alpha helix were the most,accounting for 61.78%,followed by the random coil,accounting for 20.78%.Function prediction showed that the high odds predicted from signal transducer receptor,structural protein,ion channel,immune response and stress response in oppD/F protein.The results could provide a theoretical basis for further exploration of its function of oppD/F gene of Mycoplasma bovis isolated strain.

Key words: Mycoplasma bovis; oppD/F gene; cloning; bioinformatics analysis

CLC Number:

S852.62

LING Chen, HAO Chengwu, ZHANG Fei, HOU Feng, HE Sun. Cloning and Bioinformatics Analysis of oppD/F Gene of Mycoplasma bovis Isolate Strain in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 18-27.

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Cloning and Bioinformatics Analysis of oppD/F Gene of Mycoplasma bovis Isolate Strain in Xinjiang

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