›› 2010, Vol. 37 ›› Issue (2): 75-79.

• 生物技术 • Previous Articles     Next Articles

Research on Biological Characteristics and Immunogenicity of the Molecular Marked and Virulence Deleted Vaccine Strain YZ-2 of Brucellosis

ZHANG Fu-xian1,2, LI Xiao-yan1, YANG Yan-ling1,2, ZHANG Hui1,2, 
LANG Xu-long1, TANG Jie1, WANG Xing-long1   

  1. （1.The Military Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130062, China;2.College of Animal Science and Veterinary Medicine Jilin University, Changchun 130062, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract: To determine the biological characteristics and immunogenicity of the molecular and virulence deleted vaccine strain YZ-2 of Brucellosis. The biological characteristics of Brucella abortus YZ-2 was evaluated by dyeing, toxicity, generation stability; vaccination of mice with YZ-2, the humoral immunity and cellular immunity were detected, and the results were analyzed. The research found that the biochemistry characteristics of YZ-2 and S19 were nearly identical, and the colonies and the suspension have disparity. The variation experiment proved that YZ-2 had become rough type from smooth type. Virulent experiment suggested that strain YZ-2 had lost some residual virulence of the parental strain, also YZ-2 has good heredity stability. Research verified strain YZ-2 has the immunogenicity as well as the parent strain. The results suggested that the safety of YZ-2 is better than parent strain, and the stability is well, has the same immunogenicity as parent strain; YZ-2 has good prospects to be used.

Key words: Key words: Brucella; molecular marked vaccine; YZ-2; immunogenicity