›› 2010, Vol. 37 ›› Issue (2): 71-74.

• 生物技术 • Previous Articles     Next Articles

Establishment of Protein Misfolding Cyclic Amplification for 22L PrPSc


LI Jian-jiang1,2, LI Ze-hong1, WAN Jia-yu2, GAO Hong-wei2   

  1. (1.Jilin Agricultural University, Changchun 130118, China; 2.Research Institute of Veterinary, Academy of Military Medical Sciences, Changchun 130062, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract:

Abstract: Establishing a method of PMCA for 22L strain PrPSc based on the method called protein misfolding cyclic amplification (PMCA) established by Saborio and Soto to research the biochemical mechanism of PrPSc formation. Under the existence of the anion detergent lauryl sodium sulfate (SDS), the method can directly degrade the products that producted by repeated amplification, and can in the brain tissue homogenate of hamster effectively imitate the replication mode that PrPSc run in vivo in a accelerated way. Therefore, it can amplify PrPScto high concentrations in a few hours. We found that in vitro unbolted brain tissue homogenate is more efficiently than purified reconstituted about PrPSc conversion, this discovery implied that highly efficient PrPSc conversion may associated with other unknown host factor. Researching indicated that the improved PMCA technique that we established can be used to research the biochemical mechanism of PrPSc formation.

Key words: Key words: prions; 22L strain; protein misfolding cyclic amplification