›› 2010, Vol. 37 ›› Issue (2): 66-70.

• 生物技术 • Previous Articles     Next Articles

Effects of Follicle Size on Developmental Competence of Liaoning Cashmere Goats Oocytes Following Maturation, Fertilization and Culture in vitro

TIAN Chang- yong1, YANG Jing- chao2, SONG Lian- xi1, MA Ze- fang2, WANG Xiao- song2

  

  1. （1.Department of Animal Science, Liaoning Agricultural Vocational- technical College, Yingkou 115009, China;2.College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract:

Abstract: This experiment was carried out to study effects of follicle size on collecting effect and developmental competence of goats oocytes derived from abattoir ovaries following maturation, fertilization and culture in vitro. In this experiment, the aspiration method was applied to collect the follicular oocytes. The results showed that follicle size directly affected collecting effect of oocytes and development of oocytes after IVMFC. The rate of collecting oocytes, the avaible oocyte and maturation was interrelated with the diameter of follicular positively. Both fertilization and cleavage rates of oocytes from 2.1 to 5 mm follicles were significantly higher than from >5 mm follicles and ≤2 mm follicles, which were suitable for embryo in vitro production. The oocytes from ≤2 mm follicles had the lowest developmental competence in vitro. Both fertilization and cleavage rates of oocytes from >5 mm follicles decreased ascribed to oocytes degeneration. We could improve the developmental competence of oocytes from >5 mm follicles by decreasing the cultural time in vitro.

Key words: Key words: follicle size; Liaoning cashmere goats; oocyte; culture in vitro