Abstract: Abstract：The erythrocyte agglutination assay for detection of the antibody to gE protein of pseudorabies virus in porcine was established by the anti- human red blood cell single chain fragment variable- pseudorabies virus gE bifunctional fusion protein as antigen. The optimal work concentration of antigen was 55 μg/mL. The dilution of sera was 1∶20. The reaction time of sera was 15 min. It revealed a negation reaction with positive sera of classical swine fever virus (CSFV), porcine parvovirus virus (PPV), porcine reproduction and respiratory syndrome virus (PRRS), Japanese encephalitis virus (JEV), Brucella and serum of swine inoculated by PRV gE deleted vaccines. It revealed a positive reaction with PRV standard positive serum. The total coincidence rate of the negative and positive reaction reached 100%. The results showed that the established erythrocyte agglutination assay has the advantages such as high sensibility and strong specificity and can be used to detect the porcine serum antibodies to gE protein of PRV.

Key words: Key words：pseudorabies virus; erythrocyte agglutination assay; gE gene; antibodies detection