猪流感病毒山东分离株H1N1亚型HA基因的克隆与序列分析

›› 2010, Vol. 37 ›› Issue (12): 77-81.

• 生物技术 • Previous Articles Next Articles

HA Gene Cloning and Sequence Analysis of Subtype H1N1 Swine Influenza Virus Isolated from Shandong

ZHANG Tai-xiang¹，LING Zong-shuai²，LIU Wen-peng¹，XU Biao³，LIANG Cheng-zhu³，ZHU Lai-hua³，SUN Jun¹，WANG Hong-bing¹

(1.Weifang Entry-Exit Inspection and Quaratine Bureau,Shangdong Province, Weifang 261041, China; 2.Jinan Entry-Exit Inspection and Quaratine Bureau, Jinan 250010, China; 3.Shandong Entry-Exit Inspection and Quaratine Bureau, Qingdao 250023, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-12-20 Published:2010-12-20

Abstract

Abstract: HAgene of H1N1 subtype swine influenza virus was amplified by RT-PCR, the PCR products were obtained with the pMD18-T vector, and sequenced. Homology analysis showed that HA gene nucleotide homology of the isolated strain and other H1N1 subtype swine influenza virus were 70.7% to 90.8%, the highest homology was that with the A/swine/Zhejiang/1/2007, and homology of other strains were relatively low. Phylogenetic tree analysis showed that the evolutionary relationships of HA gene isolated in Shandong and that of European lineage of swine influenza virus were closet,proved that the isolated strain may be derived from the North American and Eurasian lineages of swine influenza virus lineage restructuring.

Key words: swine influenza virus; HA gene; cloning; sequence analysis

CLC Number:

ZHANG Tai-xiang;LING Zong-shuai;LIU Wen-peng;XU Biao;LIANG Cheng-zhu;ZHU Lai-hua;SUN Jun;WANG Hong-bing. HA Gene Cloning and Sequence Analysis of Subtype H1N1 Swine Influenza Virus Isolated from Shandong[J]. , 2010, 37(12): 77-81.

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HA Gene Cloning and Sequence Analysis of Subtype H1N1 Swine Influenza Virus Isolated from Shandong

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