Abstract: In order to obtain high-yield pure plantaricin, and research on biological veterinary, plantaricin PlnE and PlnF were synthesised by Invitrogen. The aim genes were inserted into a bacterial expression vector pGEX-4T-1 to construct two prokaryotic expression vectors of pGEX-4T-E and pGEX-4T-F. After IPTG induction and express optimization, the fusion proteins were purified by affinity chromatography and detected by SDS-PAGE. The results showed that expression vectors were constructed correctly, more than 30% pure fusion proteins were obtained, which indicated peptides PlnE and PlnF could be accurately expressed in prokaryote cells, and lay a foundation for further researches on their bioactivity.

Key words: plantaricin; bacteriocin; heterologous expression; fusion protein