Abstract: 200 male Hyline Brown chicklings were divided into 4 groups(A, B,C and D), each group with 50 broilers. The group A was injected with inactivated oil-emulsion vaccines against H5N2 subtype avian influenza virus with adjuvant of astragalus polysaccharides (APS), the group B was injected with inactivated oil-emulsion vaccines against H5N2 subtype avian influenza virus with adjuvant of ginseng saponin (GS), the group C and D were injected with inactivated oil-emulsion vaccines against H5N2 subtype avian influenza virus and physiological saline(control), respectively. Five chicks were selected randomly before vaccination, the blood was taken by heart puncture and anticoagulated by EDTA.The other, each of five chicks was selected randomly from each group on the 5th, 12th and 19th days after vaccination, and the blood was also taken by heart puncture and anticoagulated by EDTA. Detecting the changes of peripheral blood T lymphocyte subgroup of each group in different stage. The data showed that the APS and GS can enhance the ratio of CD4⁺/CD8⁺ significantly compared with the common vaccine and blank groups(P<0.01), the APS and GS can also elevate the percentage of CD4⁺ subgroup, and the elevation rates were significantly higher than preimmune (P<0.01 or P<0.05).

Key words: avian influenza virus inactivated vaccines; astragalus polysaccharides; ginseng saponin; T lymphocyte subgroup