Abstract: To study the function of small molecule antibody, light chain variable genes which was amplified from species-specific monoclonal antibodies against E.acervulina was purified, and cloned into clone vector pMD18-T by gene recombination techniques. The recombinant plasmid was transformed into E.coli JM109 by screening. Several recombinants that had been inserted with the target fragments were obtained. The positive clones containing recombination were identified by the methods of the gene sequencing. The result demonstrated that light chain variable gene was 312 bp.

Key words: E.acervulina; monoclonal antibodies; variable genes; cloning; nucleotide sequencing