›› 2009, Vol. 36 ›› Issue (8): 27-29.

• 生理生化 • Previous Articles     Next Articles

Determination of Ractopamine Residues in the Swine Urine by Chemiluminescence Immunoassay

GAO Bin-wen1,2, ZHANG Su-xia1, LIANG Yong-hong2, XING Bao-song2   

  1. (1.Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2.Institute of Animal Husbandry and Veterinary Research Science, Henan Agriculture Science, Zhengzhou 450002, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-08-20 Published:2009-08-20
  • Contact: ZHANG Su-xia

Abstract: A method was established for the determination of Ractopamine (RAC) residues in swine urine by indirect competitive chemiluminescence immunoassay (CLIA). The limit of detection was 0.01 ng/mL, and the detection ranged from 0.036 to 32.2 ng/mL. At the fortified levels of 0.1, 1 and 10 ng/mL, mean recoveries was 73.2%, the intra-assay variation was 9.7%， and the inter-assay variation was 11.3%.

Key words: chemiluminescence; immunoassay; Ractopamine; swine urine

CLC Number: