China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (6): 69-72.

• 遗传繁育 • Previous Articles     Next Articles

cDNA Clone of Antimicrobial Peptide from the Reproductive Tract of Ovis aries and Construction of its Eukaryotic Expression Vector

KU Chao-feng1，2，WANG Xin-hua2，BO Xin-wen2，ZHANG Hui1，2，LV Ting-de1，2， LI Na1，2   

  1. (1.College of Animal Science & Technology, Shihezi University, Shihezi 832003, China; 2.The Breed and Biotechnology Key Laboratory of Sheep in Xinjiang Bingtuan, Shihezi 832000, China)
  • Received:2008-12-19 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
  • Contact: BO Xin-wen

Abstract: ORTAP40 is an antimicrobial peptide which is isolated from the reproductive tract of Ovis aries Chinese Merino. It plays a central role in the early innate immune defense against infection. In this study, we cloned the 3′ end full-length cDNA of ORTAP40 from reproductive tract of Ovis aries, using rapid amplification of cDNA ends (RACE) and N-terminus amino acids sequence（FJ514476）. The observed cDNA structure indicated that ORTAP40 theoretical average molecular weight for the peptide of 40 amino acid residues is 4.8 ku in agreement with reported. A mature peptide gene was synthesized according to the amino acid sequence of ORTAP40 and the biased codon usage of Pichia pastoris, the gene was 120 bp in length and was inserted into Pichia pastoris expression vector pPIC3.5K, pPICZαA by gene recombination and constructed expression plasmid pPIC3.5K-ORTAP40 and pPICZαA-ORTAP40.

Key words: reproductive tract of Ovis aries; antimicrobial peptide; cDNA clone; expression vector

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