Abstract: Using the RT-PCR technology, to detect the Guangxi Nanning, Yulin, Laibin, Guigang, Liuzhou, Qinzhou, Fangchenggang, Chongzuo, Hezhou, Guilin, Guangdong, Hainan and other parts of a dozen suspected PRRSV infection occurred in the 28 pig farms delivery 264 sick or dead pigs were seized in the lungs, spleen, lymph nodes and other organs for porcine reproductive and respiratory syndrome virus (PRRSV) detection; a total of 94 samples were positive, the positive rate was 35.6%; at the same time use of PRRSV indirect ELISA kit to detect the period from 2005 to 2007 in 48 pig farms 875 serum samples, the test results showed that 39 farms infected with PRRSV rate was 81.3%, 338 in the presence of serum positive PRRSV antibody positive rate of 38.6%. On the expected positive disease after vaccination Marc-145 cells, clear cytopathic effect (CPE), to continue to expand cultivation, harvesting and virus isolation, -70 ℃ preservation reserve. Epidemiological survey results show that the prevalence of Guangxi and the surrounding areas PRRSV infection, and the high rate of infection has become the hog industry in the region against the major diseases. 

Key words: PRRSV; seperation and identification indirect ELISA; epidemiology