Abstract: In order to explore the oxidative damage effect and the molecule mechanism of manganese on kidney protein of chicken. 500, 800, 1700 mg/kg MnCl2 were added to the forage to establish the model of the sub-chronic manganese poisoning. 2,4-dinitropheny hydrazine was used to detect the protein carbonyl content to evaluate the degree of protein oxidative damage. The results showed that the protein carbonyl content of kidney increased in each poisoned group compared with control group, and there was a time-dose relationship. Therefore， it is demonstrated that manganese could increase the protein carbonyl content, and induce oxidative damage.

Key words: manganese; chicken; carbonyl content; protein oxidative damage