›› 2009, Vol. 36 ›› Issue (2): 72-74.

• 生物技术 • Previous Articles     Next Articles

Establishment of a Loopmediated Isothermal Amplification Assay for the Detection of African Swine Fever Virus

JIANG Yanzeng,ZHU Hongfei   

  1. (Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100193,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-02-20 Published:2009-02-20
  • Contact: ZHU Hong-fei

Abstract: A rapid assay for the African swine fever virus (ASFV) by loop mediated isothermal amplification (LAMP) was established. This method was performed in a water bath at 63 ℃ without any other expansive equipment. Positive results were got in 30 min with a sensitivity 100 fold of PCR. And no crossreaction with other usual DNA virus of swine was observed. This method is used suitablely to detect ASFV in field or any other place lack of a laboratory environment.

Key words: African swine fever virus; LAMP

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