小反刍兽疫病毒间接ELISA抗体检测方法的建立与初步应用

Abstract

Abstract: This study established an indirect antibody enzyme-linked assay system based on the nucleocapsid （N） protein of peste des petits ruminants virus(PPRV).We optimized some factors such as antigen coating concentration (10 mg/L)，the dilution of serum was 1∶40，the work concentration of HRP-labeled anti-goat IgG(1∶200)，reacted conditions of samples and HRP-labeled anti-goat IgG(37 ℃，60 min).The CV is not beyond 0.1.The developed method is suitable for PPRV monitoring and detection，especially for screening large number of samples in farms.

Key words: peste des petits ruminants virus(PPRV); nucleocapsid protein; prokaryotic expression; indirect ELISA

CLC Number:

S852.65⁺9.5

JIA Feng-qin;LI Gang;LI Wei;SHI Li-jun;HU Xiao-hua. Establishment of Antibody Indirect ELISA of Peste des Petits Ruminants Virus and its Initial Application[J]. , 2009, 36(12): 149-152.

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Establishment of Antibody Indirect ELISA of Peste des Petits Ruminants Virus and its Initial Application

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