Abstract: Study on the effect of Xylazine on erythrocyte adhesion and membrane fluidity in dogs. 12 healthy dogs were selected in this experiment, were injected Xylazine 3.0 mg/kg by weight with intramuscular injection, collected the blood 5 mL from peripheral vein at the times of 0 (before injected Xylazine) and 2, 8, 24, 72, 120, 168 h after injected Xylazine, than the blood were centrifugalized to dissociate the erythrocyte, got the erythrocyte suspension and gained the erythrocyte membranes which were detected the C3b acceptor chaplet rate, immunocomplex chaplet rate and erythrocyte fluidity. The results were following:①There was a downtrend of the C3b acceptor chaplet rate at 2 h after injecting Xylazine, but the difference with the control group was not significant (P>0.05), there was highly significant difference with the control group at 8 h, since then began to restore and returned to normal at 168 h. But the immunecomplex chaplet rate showed the opposite trend, it was very significant higher than the control group at 2 to 24 h from injecting Xylazine, returned to normal at 72 h and there was not significant difference with the control group (P>0.05). ②Detected the erythrocyte membrane fluidity by fluorescence polarization, the results showed that membrane fluidity was increased, but there was not significant difference with the control group (P>0.05). The conclusions indicated that there were effect of Xylazine anaesthesia on the erythrocyte immunization adhesion and membrane fluidity in dogs, but the influences on membrane fluidity were not significant.

Key words: dogs; anaesthesia; erythrocyte; chaplet rate; membrane fluidity