O型口蹄疫病毒VP1基因在2种原核表达载体上的表达

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (10): 65-67.

• 生物技术 • Previous Articles Next Articles

Compare of O Type Foot and Mouth Disease Virus VP1 Gene in TwoProkaryotic Expression Vector

WANG Hai-feng^1,2, YI Zhong¹, WEI Yu-rong¹, Huermaxi¹, FU Zi-hua^1

(1.Veterinary Research Institute, Xinjiang Academy of Animal Sciences, Urumqi 830000, China;2.Animal Medical College, Xinjiang Agriculture University, Urumqi 830052, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: FU Zi-hua

Abstract

Abstract: The foot and mouth disease virus VP1 gene was amplified by PCR and cloned into T vector, pET-28a-VP1 was achieved through pET-28a and T vector, were dual-enzyme digested by NdeI and XhoI; pET-41a-VP1 was achieved through pET-41a and T vector, were dual-enzyme digested by EcoR I and XhoI, respectively. Then both pET-28a-VP1 and pET-41a-VP1 were transformed into BL21, respectively. When 37 ℃, different IPTG concentrations and 32 ℃, 0.01 mmol/L IPTG inducible expression, the results showed that protein yield of pET-28a-VP1 more than pET-41a-VP1, moreover status of protein was not effected by DTT and ethanol.

Key words: FMDV; VP1 gene; prokaryotic expression; vector

CLC Number:

Q785

WANG Hai-feng;YI Zhong;WEI Yu-rong;Huermaxi;FU Zi-hua. Compare of O Type Foot and Mouth Disease Virus VP1 Gene in TwoProkaryotic Expression Vector[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(10): 65-67.

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Compare of O Type Foot and Mouth Disease Virus VP1 Gene in TwoProkaryotic Expression Vector

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