蝉抗菌肽基因密码子优化及其毕赤酵母表达载体构建

›› 2009, Vol. 36 ›› Issue (10): 44-46.

• 生物技术 • Previous Articles Next Articles

Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris

LIU De-hui, HUANG Yu-mao, XU Hui, JIAO Ying

(College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: HUANG Yu-mao,

Abstract

Abstract: According to the partiality codon of Pichia pastoris, the antibacterial peptide Cryptonin gene was designed and synthesized. The modified antibacterial peptide gene was cloned into the pGAPZα-A vector to construct the recombinant expression vector pGAPZα-A-Cryptonin. Then the plasmid was identified by restriction enzyme digestion, PCR amplification and sequencing. The successfully constructed plasmid is the background for the studies on high expression and bioactivities of Cryptonin.

Key words: Cryptonin; codon optimization; pGAPZα-A vector; vector construction

CLC Number:

LIU De-hui;HUANG Yu-mao;XU Hui;JIAO Ying. Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris[J]. , 2009, 36(10): 44-46.

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Codon Optimization of Cicada Antimicrobial Peptide Gene and its Expression Vector Construction in Pichia pastoris

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