Abstract: 210 broilers randomly assigned to seven groups, with three replications per group. The control group was provided with the basal diet (not including any drug additive), yeast culture was added to the basal diet at the level of 0.1%,0.3%,0.5%,0.7%,0.9% respectively, and xanthomycin was added to the basal diet at the level of 5 mg/kg to seven experiment diets. The feeding trial started at 1 d and ended at 28 d. The experiment was conducted to study the effects of the different supplement level of yeast culture and xanthomycin on anti-oxidation and cholesterol in broilers. The results indicated that the dietary 0.5% yeast culture reduced significantly NH3 in caecum by 15.49% and 18.83%(P<0.05) respectively，when compared with the control and flavomycin；the dietary 0.5% yeast culture improved SOD by 33.52% and 28.26%(P>0.05) respectively，when compared with the control and flavomycin; the dietary 0.5% yeast culture reduced cholesterol by 26.47% and 20.89% respectively，when compared with the control and flavomycin,these data proved that the dietary yeast culture improved the serum CHOL，SOD and CHOL of chest.

Key words: yeast culture; broilers; anti-oxidation; cholesterol