采用蚀斑技术克隆纯化口蹄疫病毒的研究

China Animal Husbandry & Veterinary Medicine ›› 2008, Vol. 1 ›› Issue (12): 71-73.

• 生物技术 • Previous Articles Next Articles

Study on Cloned and Purified FootandMouth Disease Virus by Plaque Technique

LI Yujuan^1，2，WANG DianZhu³，GAO Yanhua⁴，ZHANG Shuxia¹

.College of veterinary Medicine, Northwest A & F University, Yangling 712100, China； 2.Baotou AgricultureHusbandry School,Baotou 014030， China;3.Baotou Agricultural Redamation Group Limited Liability Company, Baotou 014060， China；4.Inner Mongolia Biological Pharmaceutical Factory, Hohhot 010030, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

Abstract

Abstract: In this study, the AsiaⅠ_AKT03 strain of footandmouth disease virus(FMDV) was cloned and secondly cloned by plaque technique. The 50% of tissue culture infective doses (TCID₅₀) and 50% of lethal doses (LD₅₀) in suckling mice were compared with original, firstly cloned and secondly cloned FMDV.The results indicated that AsiaⅠ_AKT03 strain of FMDV could be successfully cloned by plaque technique. Through TCID₅₀ and LD₅₀ detection, it was found that the virulence of cloned strain was significantly higher than that of original virus. 

Key words: FMD; plaque technique; clone; purify

LI Yujuan;WANG DianZhu;GAO Yanhua;ZHANG Shuxia. Study on Cloned and Purified FootandMouth Disease Virus by Plaque Technique[J]. China Animal Husbandry & Veterinary Medicine, 2008, 1(12): 71-73.

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Study on Cloned and Purified FootandMouth Disease Virus by Plaque Technique

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