China Animal Husbandry & Veterinary Medicine ›› 2008, Vol. 1 ›› Issue (12): 65-70.

• 生物技术 • Previous Articles     Next Articles

tudies on Genetic Variation of VP1 Genes of AsiaⅠ FMDV Inactivated Vaccine Strains from Serial Passages in Different Hosts

ZHU Jingjing1,2, SHENG Zhuojun3, FU Zihua1, YI Zhong1,WEI Yurong1, HUANG Jiong1,MA Wenge1,HU Jun3,ZHU Gang4,XU Yaping4   

  1. 1. Veterinary Research Institute, Xinjiang Academy of Animal Sciences,Urumqi 830000,China；2. Xinjiang Agriculture University, Urumqi 830052,China;3. Xinjiang General Station of Animal Epidemic Prevention，Urumqi 830000，China；4. Xinjiang Tiankang Husbandary Biological Technique Company Ltd，Urumqi 830032 , China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

Abstract: The VP1 gene of FMDV contains major antigenic determinant and has the highest mutation rate in four structural proteins. Analysis of genetic variation during the passage is indispensable for vaccine research. This paper was studied on the sequence analysis of the VP1 gene of inactivated vaccine strains of FMDV which was generated in different hosts such as suckling mouse and BHK21 cell. The result demonstrated that the homologies among VP1 gene sequences were 99.4% to 99.8%，and the homologies among the deduced amino acid sequences were 98.6% to 100%. Compared with the MF1, VP1 gene and the main antigenic sites were conserved. So it is stable for producing inactivated vaccine and suitable to immune prevention of related livestock in prevailed area.

Key words: FMDV; type AsiaⅠ; strain of inactivated vaccine; VP1 gene; analysis of genetic variation