Abstract: Twenty-eight feces samples were collected from feline panleukopenia virus (FPV) cases which had got temperature high, vomiting, diarrhea with blood and dehydration. Seven feline panleukopenia virus strains were isolated from feces samples. They were named JX-1,JX-2,JX-3,JX-4,JX-5,JX-6 and JX-7. F81 cell line on which the FPV was transferred of the third culture were observed cytopathic effect (CPE) such as defluxion, disfiguration and liberation. FPV strain belonged to DNA virus by nucleic acid type analysis ,in which the metabolism of FPV strain was suppressed by 5-IUDR. The isolated virus strain could agglutinate red blood cell of porcine (agglutination titer was from 2⁶ to 2⁸) and it was inhibited by the FPV positive serum. Virion was round or hexagon, and its diameter was approximately from 20 nm to 30 nm by the electronic microscope. Animal experiment indicated the experimental kitty appeared disease about seven days after it inoculated with 1ml virus culture solution. HA and HI detections were positive from the feces sample of ill kitty.

Key words: feline panleukopenia virus; isolate; identify