青贮料中肠球菌的分离与筛选

Abstract

Abstract: This experiment was preliminary identification and screening the Enterococcus isolated from silage.Three isolates were influenced by three factor: temperature, initial pH value and concentration of rumen liquid, studied by orthogonal design. Screening the potential probiotic strains for dairy cow. The results showed that three isolates have the similar best culture condition: medium contain 2% glucose,1% peptone, 20% rumen liquid, initial pH value 5.8, 3% inoculation,40 ml liquid capacity ,fermentation temperature was 36℃,fermentation time was 24 h.Under this condition，the biomass of three isolates were 1.9907,1.2963 and 1.2752.Number 1 isolates which have the max biomass was identified to be Enterococcus Faecium by Institute of Microbiology , Chinese Academy of Sciences.

Key words: silage; enterococcus; isolation; screening

CLC Number:

S816.3

JIANG Yanmei;;WANG Jiaqi;DENG Lufang;LUO Qiujiang;BU Dengpan. Isolation and Screening of Enterococcus From Silage[J]. , 2008, 1(1): 19-21.

[1]	WU Danning, ZHANG Yanan, HUANG Tao, ZHOU Xiaolong, WANG Han, LI Xiangchen, LI Hongjun, WEN Jun, ZHANG Qi, ZHAO Ayong, YANG Songbai. Effects of Whole-plant Corn Silage on the Gut Microbiota of Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 530-539.
[2]	YAN Weiming, CHEN Yakun, YANG Pengbiao, BU Dengpan, ZHANG Jianyong, WANG Jianping, ZHAO Liansheng, ZHAO Meng. Research Progress on Quality Assessment Methods of Silage [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 135-144.
[3]	WANG Dongjing, WU Jincuomu, ZENG Jiangyong. Isolation, Identification and Biological Characteristics Analysis of Clostridium perfringens Type A from Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 229-241.
[4]	GONG Youquan, ZHOU Xiaocui, ZHENG Hui, CHEN Feng, CAO Zhenshan, SHA Zhou, ZHANG Hui, CUI Jin, WU Rui, NI Bo. Study on the Antagonism of Reboxetine Mesylate Against Senecavirus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 268-277.
[5]	DOU Junfeng, WANG Zui, LI Li, LU Qin, JIN Xinxin, LING Xiaochun, LUO Qingping, ZHAI Xinguo. Identification of Subgroups A, B and J Avian Leukosis Virus Co-infection and Sequence Analysis of gp85 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 302-311.
[6]	YANG Tingting, FENG Zijuan, HUANG Yi, ZHOU Honghong. Isolation, Identification and Biological Characteristics of Enterobacter hormaechei Phage [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 323-329.
[7]	ZHANG Lei, CHEN Liang, JIN Zhenhua, FENG Wanyu, MIAO Yan, LAN Shijie, QIN Pingwei, SHEN Sisi, LI Dan, ZHANG Bei, DONG Jiaqiang, HUANG Xuankai, LI Xuye, JIANG Botao, WANG Hongbao, SHI Tongrui. Isolation, Identification and Pathogenic Characteristics of One Pasteurella multocida Isolate from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 347-356.
[8]	WANG Yaqin, ZONG Junlin, ZHANG Tao, GUAN Weijun. Isolation,Culture and Differentiation Potential of Bovine Adipose Mesenchymal Stem Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3530-3540.
[9]	CHEN Changyi, LI Gen, HE Hongling, CHEN Yutong, HUANG Xuezhe, LUO Jun, GUO Xiaofeng. Isolation,Identification and Whole Genome Sequence Analysis of a Strain of Feline Calicivirus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3707-3718.
[10]	LI Tingting, LIU Zichao, ZHOU Yongshun, HE Ying, GENG Fanghao, CHEN Jieran, GAO Jianfeng. Effects of Two Treatment on Analysis of Mouse Dendritic Cells for Presentation of Brucella abortus Immunopeptidome in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3730-3739.
[11]	ZI Jiaxin, LIAN Yingjie, CHEN Minyi, LI Qiuxu, ZHANG Weize, LU Mengke, ZHANG Yangyang, QU Haoxuan, MA Jun, BAI Aiquan. Drug Resistance and Pathogenicity of 4 Strains of Streptococcus suis Type 2 in Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3740-3751.
[12]	SUN Yue, WANG Qi, MAO Wei, WANG Bo, DONG Haiyan, FAN Hongliang, HAO Puguo, ZHAO Hongxia. Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3811-3822.
[13]	WANG Dongjing, SU Zhonghua, YUAN Zhenjie, MA Hongcai, ZENG Jiangyong. Isolation,Identification and Biological Characteristics of Streptococcus Isolated from Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3823-3832.
[14]	CHEN Yakun, YAN Weiming, BU Dengpan, ZHAO Liansheng, XU Jianchu, SHEN Yifan, ZHENG Airong, ZHANG Jianyong. Study on Mixed Silage Quality of Calotropis gigantea and Pennisetum sinese with Different Proportions [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3065-3072.
[15]	MA Jing, YANG Danjiao, WEI Longpin, GAN Daibing, LONG Zhixin, HE Yuqi, QING Ting, FAN Yandi, ZHOU Long. Isolation,Identification and Biological Characteristics Analysis of a Moraxella pluranimalium Strain from Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3275-3285.

Isolation and Screening of Enterococcus From Silage

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments