Abstract: Intracytoplasmic sperm injection (ICSI) procedure has become the treatment of choice in cases of male factor infertility and in cases of failed conventional IVF cycles. In bovine, the technique is mainly used to produce sexed embryos and transgenic animals, and to research the mechanism of fertilization. Research status, main results and progress of the recent years were reviewed in this paper. At the same time, the main problem of bovine ICSI were expatiated. And it has also made a prospect of the application of ICSI in human and animals.

Key words: bovine; ICSI