广西罗非鱼源无乳链球菌分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2025.06.041

Abstract

Abstract: 【Objective】 The aim of this experiment was to understand the epidemic regularity and drug resistance characteristics of Streptococcus agalactiae in Guangxi,and provide scientific basis for healthy tilapia breeding.【Method】 The diseased fish from tilapia farm in Yulin,Beihai,Guigang in Guangxi were collected for bacterial isolation and culture.The isolated strains were identified by morphological observation and 16S rRNA sequencing analysis.The serotypes and main virulence genes of the isolates were identified by PCR,and their drug resistance was determined by K-B method.【Result】 A total of 38 Streptococcus strains were isolated,and the isolated strains formed milky white round small colonies with hemolytic rings on the blood plate.Gram staining was positive with typical short chain,and all were identified as Ⅰa Streptococcus agalactiae.Virulence genes sodA,cylE,gapC,bac and bca could be detected in all isolates,but virulence gene scpB could not be detected.The sensitivity of 38 isolates to aminoglycosides was low,and the resistance rate to amikacin was more than 85%.The isolates were highly sensitive to β-lactam drugs,among which penicillin G and cefoperazone were 100% sensitive.Among 38 strains of isolates,23 isolates showed multiple drug resistance,the number of drug resistance was 3-10,and 13 isolates were more than 4,accounting for 34.21% of the total isolates.【Conclusion】 The main serotype of Streptococcus agalactis derived from tilapia in Guangxi was type Ⅰa.The main virulence genes were sodA, cylE,gapC, bac and bca.The isolates were resistant to aminoglycosides,suggesting that the use of these drugs should be avoided.

Key words: tilapia; Streptococcus agalactiae; serotype; virulence gene; drug resistance

CLC Number:

QUAN Chenyu, ZHOU Yingning, PU Chanjuan, CHEN Tingting, XU Xinting, LU Bingxia, XU Yilan, ZHAO Shuo, YANG Xunye, DUAN Qunpeng, QIN Yibin, LI Bin, CHEN Zhongwei, HE Ying. Isolation,Identification and Drug Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2893-2903.

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Isolation,Identification and Drug Resistance Analysis of Streptococcus agalactiae from Tilapia in Guangxi

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