谷氨酰胺对肠产毒素性大肠杆菌感染小鼠肠道损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2023.10.007

Abstract

Abstract: 【Objective】 This experiment was conducted to investigate the protective effect of glutamine (Gln) on intestinal damage in mice infected with enterotoxigenic Escherichia coli (ETEC),and provide a theoretical basis and rationale for Gln to alleviate E.coli intestinal injury.【Method】 Twenty-four health SPF KM mice were randomly allocated to 3 groups:Control group (Con),model group (ETEC) and Gln intervention group (ETEC+Gln).Mice in Con and ETEC groups were given 0.2 mL saline daily by gavage,and mice in ETEC+Gln group were given 0.2 mL 1% Gln.On days 7,the mice in ETEC and ETEC+Gln groups were challenged with 0.2 mL 8.6×10⁹ CFU/mL ETEC K88 by gavage.The trial lasted for 10 days.Mice were weighed daily.The pathological changes of jejunum were observed by hematoxylin-eosin (HE) and periodic acid-schiff (PAS) staining.The concentrations of serum inflammatory factors were determined by ELISA.The mRNA and protein expressions of inflammatory factors (IL-1β,IL-6,TNF-α and IFN-γ),tight junction proteins (Occludin,Claudin-1 and ZO-1),intestinal epithelial cell proliferation (TGF-β1,PCNA and EGF) and apoptosis (Bax and Bcl-2) in jejunum were detected by Real-time quantitative PCR and Western blotting,respectively.The protein expression of TLR4 and NF-κB p65 in jejunum were detected by immunohistochemistry.【Result】 Compared with Con group,the body weight of mice in ETEC group was significantly decreased (P＜0.05),while Gln treatment prominently reversed that.The spleen index of mice in ETEC group was significantly higher than that in Con and ETEC+Gln groups (P＜0.05).Compared with ETEC group,treatment with Gln significantly improved the villus height,the ratio of villus height/crypt depth,the number of goblet cells and the mucus thickness in jejunum (P＜0.05).Compared with Con and ETEC+Gln groups,the IL-1β and TNF-α contents,D-lac concentration and DAO activity in serum of mice in ETEC group were significantly increased (P＜0.05).Compared with ETEC group,treatment with Gln significantly decreased the mRNA expressions of IL-1β,IL-6,TNF-α,IFN-γ,TLR4,NF-κB p65 and Bax,and significantly increased the mRNA and protein expressions of Occludin,Claudin-1,ZO-1,Bcl-2,PCNA and TGF-β1 in jejunum (P＜0.05).【Conclusion】 Gln alleviated ETEC K88-induced intestinal damage of mice by restoring intestinal barrier integrity,suppressing inflammatory responses and promoting epithelial cells proliferation.

Key words: glutamine(Gln); enterotoxigenic Escherichia coli (ETEC) K88; intestinal inflammation; intestinal barrier

CLC Number:

S852.61⁺2

ZHANG Na, GUO Huijun, QIU Jianhua. Protective Effect of Glutamine on Intestinal Damage in Mice Infected with Enterotoxigenic Escherichia coli[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 3939-3949.

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Protective Effect of Glutamine on Intestinal Damage in Mice Infected with Enterotoxigenic Escherichia coli

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