China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (9): 3688-3694.doi: 10.16431/j.cnki.1671-7236.2023.09.025

• Genetics and Breeding • Previous Articles     Next Articles

Morphological and Spermatogenic Epithelial Cells of Testis of Mongolian Horse at Different Ages

LIU Yuanyi, JIA Zijie, LI Xinyu, ZHANG Lei, BOU Gerelchimeg, MANG Lai, DU Ming   

  1. Key Laboratory of Genetics Breeding and Reproduction of Horse Genusin Inner Mongolia Autonomous Region, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China
  • Received:2023-02-09 Online:2023-09-05 Published:2023-08-24

Abstract: 【Objective】 This study was aimed to explore the morphological changes of testis and the number of spermatogenic epithelial cells at different ages of Mongolian horses,so as to provide theoretical reference for the study of spermatogenesis of Mongolian horses.【Method】 Testis tissues of Mongolian horses aged 1,2,3,4,5 and 6 years old were collected,and morphological observation was made on testis tissues and convoluted spermatogenic epithelium structures by HE staining,and relevant parameters of convoluted spermatogenic epithelium cells were collected to explore their development rules.【Result】 Testis histological observation showed that there was no mature sperm in the testis tissue of the 1 year old Mongolian horse,and the lumen of the fine ductus was solid with small diameter.At the age of 2 years old,a small amount of mature sperm appeared,and the curved spermatozoa part showed a cavity structure.The number of mature sperm gradually increased with the age of Mongolian horse.The diameter and area of seminiferous tubule and the number of spermatogenic cells and Sertoli cells increased with age,and reached the highest level at 6 years old,and was extremely significantly higher than that at 1 year old (P<0.01).【Conclusion】 The Mongolian horse reached the stage of sexual maturity at 2 years old,and with the continuous progress of spermatogenesis,various spermatogenic epithelial cells began to appear,the area and diameter of seminiferous tubule of testis,the number of spermatogenic cells and Sertoli cells increased with age.The results provided theoretical reference for determining the breeding cycle and improving the breeding performance of Mongolian horse.

Key words: Mongolian horse; testicles; seminiferous tubules; spermatogenic epithelial cells

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