蒙古马肠道细菌总DNA提取方法的比较

doi:10.16431/j.cnki.1671-7236.2015.02.041

Abstract

Abstract: In this study,we extracted total DNA from fresh fecal collected from Mongolian horses through phenol-chloroform technique and two bacterial genomic DNA extraction Kits.To find more suitable methods,we had compared the concentration and purity of DNA and the landscape of amplifying the 16S rDNA V3 region.The results showed that all three methods can be used for DNA extraction from gut bacterial in Mongolian horse.Meanwhile,the quality and purity of DNA extracted by B Kit was the most effective which could be more suitable for the extraction of genomic DNA from gut bacterial and the subsequent analysis of the diversity of bacterial population and biology experiments in Mongolian horse.

Key words: Mongolian horse; intestinal bacteria; total DNA extraction; comparison of methods

CLC Number:

Q523

Yirugeletu, YANG Li-hua, ZHAO Yi-ping, REN Xiu-juan, BAI Dong-yi, ZHAO Qi-nan, MANG Lai. Comparing the Methods of Total DNA Extraction from Intestinal Bacteria in Mongolian Horse[J]. , 2015, 42(2): 498-502.

References

[1] 赵一萍,芒来.中国蒙古马遗传资源的保护与利用[J].国际遗传杂志,2010,33(4):218-221.
[2] 赵红艳,孙泽威.马属动物肠道微生物的研究进展[J].经济动物学报,2014,18(1):59-62.
[3] Islam M R,Sultana T,Melvin-Joe M,et al.Comparisons of direct extraction methods of microbial DNA from different paddy soils[J].Saudi Journal of Biological Sciences,2012,19:337-342.
[4] 夏乐先,孙文娟.煮沸裂解法和试剂盒法提取浸矿菌基因组DNA的比较[J].现代生物医学进展,2014,14(1):31-35.
[5] 李红,周友兵,陈炳耀,等.分子生物学技术在微生物多样性研究中的应用[J].河北大学学报,2003,23(4):450-453.
[6] 郭丽,赵虹.两种全血基因组 DNA提取法的比较[J].中国优生与遗传杂志,2013,21(12):59-60.
[7] 赵健元,李进华.一种高效的哺乳动物粪便DNA提取通用方法[J].激光生物学报,2008,17(5):695-700.
[8] 文红秀,李昌平. 家兔盲肠微生物总DNA的提取[J].动物科技,2004,21(12):28-29.
[9] Mcorist A L,Jackson M,Bird A R.A comparison of five methods for extraction of bacterial DNA from human fecal samples[J].Journal of Microbiological Methods,2002,50(2):131-139.
[10] Li M,Gong J H,Cottrill M,et al.Evaluation of QIAamp^ DNA Stool Mini Kit for ecological studies of gut microbiota [J].Journal of Microbiological Methods,2003,54(1):13-20.
[11] 梁金花,杨景云.两种提取肠道微生物总DNA方法的比较[J].中国微生态学杂志,2010,22(5):477-478.
[12] 黄进勇,岳彩鹏,周伟.麦田土壤细菌群落16S rDNA V3片段PCR产物的DGGE分析[J].河南农业大学学报,2007,41(4):396-400.
[13] 骞宇,赵欣.大鼠粪便中细菌基因组DNA提取方法的比较[J].食品工业科技,2014,4:166-169.
[14] 曾志光,王红宁.猪粪样DNA提取方法的比较[J].中国兽医杂志,2009,45(8):17-19.
[15] Lee Y K,Kim H W,Liu C L,et al.A simple method for DNA extraction from marine bacterie that produce extracellular materials[J].Journal of Microbiological Methods,2003,52(2):245-250.

Comparing the Methods of Total DNA Extraction from Intestinal Bacteria in Mongolian Horse

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 6

Recommended Articles

Metrics

Comments

[1]	TIAN Shuyue, SHEN Yingchao, WANG Xisheng, WANG Min, YI Minna, Tseweendolmaa, TAO Li, ZHAO Bilig, Manglai, Gerelchimeg. Study on Establishment of Immortalized Cell System of Equine Fetal Fibroblasts in Mongolian Horse [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 46-57.
[2]	SONG Lianjie, CUI Yingying, ZHAO Yiping, BAI Dongyi, REN Xiujuan, TE Rigele, DUGARJAVIIN Manglai, LI Bei. Isolation,Culture and Identification of Testis Sertoli Cells in Mongolian Horses in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2751-2758.
[3]	HAI Tang, HUANG Jin-long, ZHAO Yi-ping, HAN Hai-ge, SA Ru-la, MANG Lai. Analysis of Genetic Diversity and Phylogeny of the Mitochondrial Cytochrome b Gene of Mongolian Horses and Mongolian Wild Horse [J]. , 2014, 41(6): 162-167.
[4]	SUN Yao-gui, ZHANG Xiang-jie, CHENG Jia, WANG Jun-dong. Effects of Two Chinese Medicinal Ingredients on Growth Performance, Digestive Utilization Ratios of Nutrients, Digestive Enzyme Activity and Intestinal Bacterial in Broilers [J]. , 2013, 40(4): 93-98.
[5]	ZHAO Chun-miao, XU Chun-hou. Review on Denaturing Gradient Gel Electrophoresis and its Application in Animal Intestinal Bacterial Community [J]. , 2012, 39(7): 62-65.
[6]	YAN Bing-bing;LV Ai-jun;LUO Wei-jie;HU Xiu-cai. Isolation, Identification and Antimicrobial Susceptibility Test of Bacteria from Intestine in Megalobrama amblycephala [J]. , 2010, 37(12): 164-166.