不同断奶日龄羔羊瘤胃组织显微及超微结构比较

doi:10.16431/j.cnki.1671-7236.2021.06.010

Abstract

Abstract: The purpose of the experiment was to compare the differences of rumen tissue structure,especially the changes of ultrastructure of ruminal papillae in Hu lambs at different weaning age,so as to provide the basis for determining the suitable weaning age of Hu lambs.Twenty-four newborns of Hu sheep with birth weight of 2.80 kg±0.08 kg were randomly divided into three groups,8 lambs in each group,weaning at 30 days old (EEW-30),45 days old (EW-45) and 60 days old (NW-60),respectively.Four lambs in each group were slaughtered at corresponding weaning time and at the age of 75 days,respectively.The body weight and rumen weight were measured,and the ruminal relative weight was calculated.The rumen was sampled for morphological measurement.The results showed that:①The body weight of lambs at weaning time in EEW-30 group was extremely lower than that in NW-60 group (P<0.01),but there was no significant difference between EW-45 and NW-60 groups (P>0.05),and neither was any significant difference of the body weight among 3 groups at 75 days old (P>0.05).The change of the ruminal relative weight in 3 groups was in accord with that of the body weight.②Under the light microscope,the tunica mucosa thickness of rumen in EEW-30 group was significantly lower than NW-60 group (P<0.01),and there was no significant difference between EW-45 group and NW-60 group (P>0.05) at weaning age.At 75 days,the tunica mucosa thickness of rumen in EW-45 group was significantly higher than that in NW-60 group (P<0.05).At weaning age,the rumen papillae of EEW-30 group was extremely significant shorter than that in NW-60 group (P<0.01),and EW-45 group was also significantly shorter than NW-60 group (P<0.05).However,there was no significant difference in the width and area of rumen papilla at different weaning time and at day 75 (P>0.05).③Under scanning electronic microscope,the number of ruminal micro papillae structure per μm² at weaning age in EEW-30 group was extremely significantly lower than that in NW-60 group (P<0.01),but there was no significant difference between EW-45 group and NW-60 group (P>0.05).At the age of 75 days,there was no significant difference in the number of ruminal micro-papillae structure per μm² among the three groups (P>0.05).The results showed that there was significant differences in ruminal morphology between EEW-30 and NW-60 groups at weaning age,while EW-45 and NW-60 groups were more similar,and the change trend of ultra-microstructure of ruminal micro-papillae was the same.It was suggested that weaning at 45 days of age was more appropriate.

Key words: Hu lamb; weaning; rumen; ruminal papilla; ultra-microstructure

CLC Number:

S826

ZHAO Yaxuan, WANG Yan, AYINUER MUSA, CHEN Zhuo, YANG Ningzhi, SUN Yawei, MA Xuelian, LI Na, WANG Jinquan, ZHAO Hongqiong, YANG Huiguo, ZHAI Shaohua, YAO Gang. Comparative Study on Ruminal Histo-ultra Microstructure of Lambs Weaned at Different Time[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1976-1984.

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Comparative Study on Ruminal Histo-ultra Microstructure of Lambs Weaned at Different Time

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