塔里木马鹿干旱环境适应相关基因的筛选、克隆及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2021.06.003

Abstract

Abstract: In order to explore the structural and functions of the genes related to the adaptation of Tarim red deer(Cervus elaphus yarkandensis) to the arid environment.The sequence of thioredoxin dependent peroxidase 3 (PRDX3) gene of Tarim red deer was screened from the results of whole genome resequencing,and analyzed the expression of this gene in different tissues of Tarim red deer.The CDS sequence of PRDX3 gene of Tarim red deer was cloned and sequenced.Homology comparison,phylogenetic tree construction and bioinformatics analysis were carried out by using related software.The results showed that the expression level of PRDX3 gene in kidney was extremely significantly higher than that in lung and liver (P<0.01).The CDS region of PRDX3 gene of Tarim red deer was 660 bp in length and encoded 220 amino acids.The bioinformatics analysis using related software showed that Tarim red deer and Odocoileus virginianus (GenBank accession No.:XM_020875097.1) had the highest homology and the closest genetic distance,and with Rattus norvegicus (GenBank accession No.:NM_022540.1) had the lowest homology and the longest genetic distance.The protein molecular weight of PRDX3 in Tarim red deer was 24.42 ku and composed of 220 amino acids.It was predicted that the instability index,theoretical isoelectric point,lipid solubility index and hydrophilic average coefficient of PRDX3 protein was 25.36,5.82,85.50,and -0.05,respectively.There was O-glycosylation site and phosphorylation sites in PRDX3,but had no transmembrane region,N-glycosylation site and signal peptide,most likely located in the mitochondria.The secondary and tertiary structures of PRDX3 protein were mainly comprised of α-helix and random coil.PRDX3 protein contained the conserved domain of PRX_Typ2cys superfamily,which had relatively strong interaction with a variety of proteins.The results of this study laid a foundation for the following functional gene research of Tarim red deer.

Key words: Tarim red deer; drought adaptation; PRDX3 gene; bioinformatics analysis

CLC Number:

MAMAT Tayerjan, ELI Subinur, ABABAIKERI Buweihailiqiemu, HALIK Mahmut. Screening,Cloning and Bioinformatics Analysis of Drought Adaptation Related Genes in Tarim Red Deer[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1906-1917.

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Screening,Cloning and Bioinformatics Analysis of Drought Adaptation Related Genes in Tarim Red Deer

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